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nb100  (Novus Biologicals)


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    Structured Review

    Novus Biologicals nb100
    Nb100, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nb100+355/RPE65+Antibody+(401%2E8B11%2E3D9)+-+BSA+Free/pmc12995699-8-7-3
    Average 95 stars, based on 45 article reviews
    nb100 - by Bioz Stars, 2026-10
    95/100 stars

    Images

    Related Articles

    Transfection:

    Article Title: Sequential Fabrication of a Three-Layer Retina-like Structure
    Article Snippet: Mouse anti-RPE65 , 1:100 , NB100-355 , Novus.

    Article Title: A novel coculture model of porcine central neuroretina explants and retinal pigment epithelium cells
    Article Snippet: Retinal pigment epithelium-specific 65 kDa protein (RPE65) , Mouse monoclonal , Novus Biologicals, #NB100–355 (Cambridge, UK) , 1:50.

    Article Title: Retinal Pigment Epithelial Cell Line with Fast Differentiation and Improved Barrier Properties
    Article Snippet: RPE65 , NB100-355, Novus Biologicals , 1:100 , goat anti-mouse AF594 , A-11032 Thermo Fischer Scientific , 1:1000.

    Article Title: Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration
    Article Snippet: RPE65 , Novus Biologicals , NB100-355, RRID: AB_10002148.

    Construct:

    Article Title: Sequential Fabrication of a Three-Layer Retina-like Structure
    Article Snippet: Mouse anti-RPE65 , 1:100 , NB100-355 , Novus.

    Article Title: A novel coculture model of porcine central neuroretina explants and retinal pigment epithelium cells
    Article Snippet: Retinal pigment epithelium-specific 65 kDa protein (RPE65) , Mouse monoclonal , Novus Biologicals, #NB100–355 (Cambridge, UK) , 1:50.

    Article Title: Retinal Pigment Epithelial Cell Line with Fast Differentiation and Improved Barrier Properties
    Article Snippet: RPE65 , NB100-355, Novus Biologicals , 1:100 , goat anti-mouse AF594 , A-11032 Thermo Fischer Scientific , 1:1000.

    Article Title: Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration
    Article Snippet: RPE65 , Novus Biologicals , NB100-355, RRID: AB_10002148.

    Mutagenesis:

    Article Title: Sequential Fabrication of a Three-Layer Retina-like Structure
    Article Snippet: Mouse anti-RPE65 , 1:100 , NB100-355 , Novus.

    Article Title: A novel coculture model of porcine central neuroretina explants and retinal pigment epithelium cells
    Article Snippet: Retinal pigment epithelium-specific 65 kDa protein (RPE65) , Mouse monoclonal , Novus Biologicals, #NB100–355 (Cambridge, UK) , 1:50.

    Article Title: Retinal Pigment Epithelial Cell Line with Fast Differentiation and Improved Barrier Properties
    Article Snippet: RPE65 , NB100-355, Novus Biologicals , 1:100 , goat anti-mouse AF594 , A-11032 Thermo Fischer Scientific , 1:1000.

    Article Title: Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration
    Article Snippet: RPE65 , Novus Biologicals , NB100-355, RRID: AB_10002148.

    Generated:

    Article Title: Sequential Fabrication of a Three-Layer Retina-like Structure
    Article Snippet: Mouse anti-RPE65 , 1:100 , NB100-355 , Novus.

    Article Title: A novel coculture model of porcine central neuroretina explants and retinal pigment epithelium cells
    Article Snippet: Retinal pigment epithelium-specific 65 kDa protein (RPE65) , Mouse monoclonal , Novus Biologicals, #NB100–355 (Cambridge, UK) , 1:50.

    Article Title: Retinal Pigment Epithelial Cell Line with Fast Differentiation and Improved Barrier Properties
    Article Snippet: RPE65 , NB100-355, Novus Biologicals , 1:100 , goat anti-mouse AF594 , A-11032 Thermo Fischer Scientific , 1:1000.

    Article Title: Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration
    Article Snippet: RPE65 , Novus Biologicals , NB100-355, RRID: AB_10002148.

    Recombinant:

    Article Title: Sequential Fabrication of a Three-Layer Retina-like Structure
    Article Snippet: Mouse anti-RPE65 , 1:100 , NB100-355 , Novus.

    Article Title: A novel coculture model of porcine central neuroretina explants and retinal pigment epithelium cells
    Article Snippet: Retinal pigment epithelium-specific 65 kDa protein (RPE65) , Mouse monoclonal , Novus Biologicals, #NB100–355 (Cambridge, UK) , 1:50.

    Article Title: Retinal Pigment Epithelial Cell Line with Fast Differentiation and Improved Barrier Properties
    Article Snippet: RPE65 , NB100-355, Novus Biologicals , 1:100 , goat anti-mouse AF594 , A-11032 Thermo Fischer Scientific , 1:1000.

    Article Title: Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration
    Article Snippet: RPE65 , Novus Biologicals , NB100-355, RRID: AB_10002148.

    Virus:

    Article Title: Sequential Fabrication of a Three-Layer Retina-like Structure
    Article Snippet: Mouse anti-RPE65 , 1:100 , NB100-355 , Novus.

    Article Title: A novel coculture model of porcine central neuroretina explants and retinal pigment epithelium cells
    Article Snippet: Retinal pigment epithelium-specific 65 kDa protein (RPE65) , Mouse monoclonal , Novus Biologicals, #NB100–355 (Cambridge, UK) , 1:50.

    Article Title: Retinal Pigment Epithelial Cell Line with Fast Differentiation and Improved Barrier Properties
    Article Snippet: RPE65 , NB100-355, Novus Biologicals , 1:100 , goat anti-mouse AF594 , A-11032 Thermo Fischer Scientific , 1:1000.

    Article Title: Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration
    Article Snippet: RPE65 , Novus Biologicals , NB100-355, RRID: AB_10002148.

    Produced:

    Article Title: Sequential Fabrication of a Three-Layer Retina-like Structure
    Article Snippet: Mouse anti-RPE65 , 1:100 , NB100-355 , Novus.

    Article Title: A novel coculture model of porcine central neuroretina explants and retinal pigment epithelium cells
    Article Snippet: Retinal pigment epithelium-specific 65 kDa protein (RPE65) , Mouse monoclonal , Novus Biologicals, #NB100–355 (Cambridge, UK) , 1:50.

    Article Title: Retinal Pigment Epithelial Cell Line with Fast Differentiation and Improved Barrier Properties
    Article Snippet: RPE65 , NB100-355, Novus Biologicals , 1:100 , goat anti-mouse AF594 , A-11032 Thermo Fischer Scientific , 1:1000.

    Article Title: Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration
    Article Snippet: RPE65 , Novus Biologicals , NB100-355, RRID: AB_10002148.

    Sequencing:

    Article Title: Sequential Fabrication of a Three-Layer Retina-like Structure
    Article Snippet: Mouse anti-RPE65 , 1:100 , NB100-355 , Novus.

    Article Title: A novel coculture model of porcine central neuroretina explants and retinal pigment epithelium cells
    Article Snippet: Retinal pigment epithelium-specific 65 kDa protein (RPE65) , Mouse monoclonal , Novus Biologicals, #NB100–355 (Cambridge, UK) , 1:50.

    Article Title: Retinal Pigment Epithelial Cell Line with Fast Differentiation and Improved Barrier Properties
    Article Snippet: RPE65 , NB100-355, Novus Biologicals , 1:100 , goat anti-mouse AF594 , A-11032 Thermo Fischer Scientific , 1:1000.

    Article Title: Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration
    Article Snippet: RPE65 , Novus Biologicals , NB100-355, RRID: AB_10002148.

    Purification:

    Article Title: Sequential Fabrication of a Three-Layer Retina-like Structure
    Article Snippet: Mouse anti-RPE65 , 1:100 , NB100-355 , Novus.

    Article Title: A novel coculture model of porcine central neuroretina explants and retinal pigment epithelium cells
    Article Snippet: Retinal pigment epithelium-specific 65 kDa protein (RPE65) , Mouse monoclonal , Novus Biologicals, #NB100–355 (Cambridge, UK) , 1:50.

    Article Title: Retinal Pigment Epithelial Cell Line with Fast Differentiation and Improved Barrier Properties
    Article Snippet: RPE65 , NB100-355, Novus Biologicals , 1:100 , goat anti-mouse AF594 , A-11032 Thermo Fischer Scientific , 1:1000.

    Article Title: Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration
    Article Snippet: RPE65 , Novus Biologicals , NB100-355, RRID: AB_10002148.

    Size-exclusion Chromatography:

    Article Title: Sequential Fabrication of a Three-Layer Retina-like Structure
    Article Snippet: Mouse anti-RPE65 , 1:100 , NB100-355 , Novus.

    Article Title: A novel coculture model of porcine central neuroretina explants and retinal pigment epithelium cells
    Article Snippet: Retinal pigment epithelium-specific 65 kDa protein (RPE65) , Mouse monoclonal , Novus Biologicals, #NB100–355 (Cambridge, UK) , 1:50.

    Article Title: Retinal Pigment Epithelial Cell Line with Fast Differentiation and Improved Barrier Properties
    Article Snippet: RPE65 , NB100-355, Novus Biologicals , 1:100 , goat anti-mouse AF594 , A-11032 Thermo Fischer Scientific , 1:1000.

    Article Title: Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration
    Article Snippet: RPE65 , Novus Biologicals , NB100-355, RRID: AB_10002148.

    Clone Assay:

    Article Title: Sequential Fabrication of a Three-Layer Retina-like Structure
    Article Snippet: Mouse anti-RPE65 , 1:100 , NB100-355 , Novus.

    Article Title: A novel coculture model of porcine central neuroretina explants and retinal pigment epithelium cells
    Article Snippet: Retinal pigment epithelium-specific 65 kDa protein (RPE65) , Mouse monoclonal , Novus Biologicals, #NB100–355 (Cambridge, UK) , 1:50.

    Article Title: Retinal Pigment Epithelial Cell Line with Fast Differentiation and Improved Barrier Properties
    Article Snippet: RPE65 , NB100-355, Novus Biologicals , 1:100 , goat anti-mouse AF594 , A-11032 Thermo Fischer Scientific , 1:1000.

    Article Title: Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration
    Article Snippet: RPE65 , Novus Biologicals , NB100-355, RRID: AB_10002148.

    Software:

    Article Title: Sequential Fabrication of a Three-Layer Retina-like Structure
    Article Snippet: Mouse anti-RPE65 , 1:100 , NB100-355 , Novus.

    Article Title: A novel coculture model of porcine central neuroretina explants and retinal pigment epithelium cells
    Article Snippet: Retinal pigment epithelium-specific 65 kDa protein (RPE65) , Mouse monoclonal , Novus Biologicals, #NB100–355 (Cambridge, UK) , 1:50.

    Article Title: Retinal Pigment Epithelial Cell Line with Fast Differentiation and Improved Barrier Properties
    Article Snippet: RPE65 , NB100-355, Novus Biologicals , 1:100 , goat anti-mouse AF594 , A-11032 Thermo Fischer Scientific , 1:1000.

    Article Title: Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration
    Article Snippet: RPE65 , Novus Biologicals , NB100-355, RRID: AB_10002148.



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    Localization of EFA6A in the mouse retina (A) Immunolabelling of wild-type retinas using an anti-EFA6A antibody revealed specific EFA6A expression in the RPE, photoreceptors (both inner and outer segments), certain cells in the inner nuclear and plexiform layers, and the ganglion cell layer. Incubation with the antibody preabsorbed with purified EFA6A protein served as a negative control and confirmed labeling specificity. Scale bar 20 μm. (B) In situ hybridization using an EFA6A antisense probe, with a sense probe as control, showed specific labeling in the ganglion cell layer, inner and outer nuclear layers, and RPE. Diaminobenzidine (DAB) was used for signal detection. Scale bar 20 μm. (C) Confocal immunofluorescence microscopy demonstrates the co-localization of EFA6A with various retinal markers. Co-labelling with peanut agglutinin (upper panels) indicates EFA6A presence in the inner and outer segments of cone photoreceptors (arrows). Co-labelling with anti-rhodopsin antibodies (middle panels) revealed EFA6A expression in the outer segments, particularly in the CC (arrows). Co-labelling <t>with</t> <t>anti-RPE65</t> antibodies (lower panels) confirmed EFA6A expression in the RPE (arrows). Scale bars, 50 μm (upper panels), 25 μm (middle and lower panels).
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    Image Search Results


    Localization of EFA6A in the mouse retina (A) Immunolabelling of wild-type retinas using an anti-EFA6A antibody revealed specific EFA6A expression in the RPE, photoreceptors (both inner and outer segments), certain cells in the inner nuclear and plexiform layers, and the ganglion cell layer. Incubation with the antibody preabsorbed with purified EFA6A protein served as a negative control and confirmed labeling specificity. Scale bar 20 μm. (B) In situ hybridization using an EFA6A antisense probe, with a sense probe as control, showed specific labeling in the ganglion cell layer, inner and outer nuclear layers, and RPE. Diaminobenzidine (DAB) was used for signal detection. Scale bar 20 μm. (C) Confocal immunofluorescence microscopy demonstrates the co-localization of EFA6A with various retinal markers. Co-labelling with peanut agglutinin (upper panels) indicates EFA6A presence in the inner and outer segments of cone photoreceptors (arrows). Co-labelling with anti-rhodopsin antibodies (middle panels) revealed EFA6A expression in the outer segments, particularly in the CC (arrows). Co-labelling with anti-RPE65 antibodies (lower panels) confirmed EFA6A expression in the RPE (arrows). Scale bars, 50 μm (upper panels), 25 μm (middle and lower panels).

    Journal: iScience

    Article Title: EFA6A regulates retinal function through the control of photoreceptor cell activity and structure

    doi: 10.1016/j.isci.2026.115179

    Figure Lengend Snippet: Localization of EFA6A in the mouse retina (A) Immunolabelling of wild-type retinas using an anti-EFA6A antibody revealed specific EFA6A expression in the RPE, photoreceptors (both inner and outer segments), certain cells in the inner nuclear and plexiform layers, and the ganglion cell layer. Incubation with the antibody preabsorbed with purified EFA6A protein served as a negative control and confirmed labeling specificity. Scale bar 20 μm. (B) In situ hybridization using an EFA6A antisense probe, with a sense probe as control, showed specific labeling in the ganglion cell layer, inner and outer nuclear layers, and RPE. Diaminobenzidine (DAB) was used for signal detection. Scale bar 20 μm. (C) Confocal immunofluorescence microscopy demonstrates the co-localization of EFA6A with various retinal markers. Co-labelling with peanut agglutinin (upper panels) indicates EFA6A presence in the inner and outer segments of cone photoreceptors (arrows). Co-labelling with anti-rhodopsin antibodies (middle panels) revealed EFA6A expression in the outer segments, particularly in the CC (arrows). Co-labelling with anti-RPE65 antibodies (lower panels) confirmed EFA6A expression in the RPE (arrows). Scale bars, 50 μm (upper panels), 25 μm (middle and lower panels).

    Article Snippet: Rabbit anti-RPE65 , Novus Biologicals , Cat# NB100-355; RRID: AB_10003038.

    Techniques: Expressing, Incubation, Purification, Negative Control, Labeling, In Situ Hybridization, Control, Immunofluorescence, Microscopy